The CSF-1 receptor fashions the intestinal stem cell niche

► CSF-1R KO eliminates stem and Paneth Cell marker CD24 in the stem cell niche. ► The KO defect is cell intrinsic as shown by organoid cultures. ► Intestinal-specific CSF-1R KO leads to a progressive elimination of Paneth cells. ► This PC loss impacts intestinal stem cell gene expression. ► CSF-1R signaling pathway defects may underpin GI disorders.