High-throughput karyotyping of human pluripotent stem cells

► We report a novel high-throughput karyotyping assay for human pluripotent cells. ► This assay provides fast and easy tool for detection of chromosomal abnormalities. ► The analysis can be run from low DNA amounts with simple data analysis interface. ► The results are in good concordance with the data from G-banding and SNP arrays. ► The assay enables routine screening of stem cell lines in a cost-efficient manner.