hESC derived neuro-epithelial rosettes recapitulate early mammalian neurulation events; an in vitro model

► MKLP+ particles were observed in aggregations in the lumen of “early” rosette structures. ► MKLP+ particle aggregation is lost from the lumen of the rosettes, analogous to the developing neural tube ► MKLP+ particles are lost in “late” as opposed to “early” stage neuro--‐epithelial rosettes ► Disrupting the polarity of early NE cells ablates lumenal MKLP+ particle aggregation ► hESC--‐rosettes can model in vivo neurulation, such as the floor plate to neural tube transition