Promotion of hematopoietic differentiation from mouse induced pluripotent stem cells by transient HoxB4 transduction

► We use adenovirus vectors to transiently transduce HoxB4 gene into ES and iPS cells. ► Transient HoxB4 transduction results in efficient proliferation on stromal cells. ► Colony-forming cells are efficiently generated by transient HoxB4 transduction. ► Transient HoxB4 expression is sufficient to promote hematopoietic differentiation.