کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10891419 | 1082029 | 2013 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Polysome profiling shows extensive posttranscriptional regulation during human adipocyte stem cell differentiation into adipocytes
ترجمه فارسی عنوان
پروفیل پلیسوم نشان می دهد که تنظیم مقررات پس از ترانسکتیک در تمایز سلول های بنیادی آدیپوسیت انسان به آدیپوسیت ها
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
چکیده انگلیسی
Adipocyte stem cells (hASCs) can proliferate and self-renew and, due to their multipotent nature, they can differentiate into several tissue-specific lineages, making them ideal candidates for use in cell therapy. Most attempts to determine the mRNA profile of self-renewing or differentiating stem cells have made use of total RNA for gene expression analysis. Several lines of evidence suggest that self-renewal and differentiation are also dependent on the control of protein synthesis by posttranscriptional mechanisms. We used adipogenic differentiation as a model, to investigate the extent to which posttranscriptional regulation controlled gene expression in hASCs. We focused on the initial steps of differentiation and isolated both the total mRNA fraction and the subpopulation of mRNAs associated with translating ribosomes. We observed that adipogenesis is committed in the first days of induction and three days appears as the minimum time of induction necessary for efficient differentiation. RNA-seq analysis showed that a significant percentage of regulated mRNAs were posttranscriptionally controlled. Part of this regulation involves massive changes in transcript untranslated regions (UTR) length, with differential extension/reduction of the 3â²UTR after induction. A slight correlation can be observed between the expression levels of differentially expressed genes and the 3â²UTR length. When we considered association to polysomes, this correlation values increased. Changes in the half lives were related to the extension of the 3â²UTR, with longer UTRs mainly stabilizing the transcripts. Thus, changes in the length of these extensions may be associated with changes in the ability to associate with polysomes or in half-life.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Stem Cell Research - Volume 11, Issue 2, September 2013, Pages 902-912
Journal: Stem Cell Research - Volume 11, Issue 2, September 2013, Pages 902-912
نویسندگان
Lucia Spangenberg, Patricia Shigunov, Ana Paula R. Abud, Axel R. Cofré, Marco A. Stimamiglio, Crisciele Kuligovski, Jaiesa Zych, Andressa V. Schittini, Alexandre Dias Tavares Costa, Carmen K. Rebelatto, Paulo R.S. Brofman, Samuel Goldenberg,