کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10940879 | 1095527 | 2015 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Frequent expression of zinc-finger protein ZNF165 in human urinary bladder transitional cell carcinoma
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کلمات کلیدی
UBCqRTPCRTURBTCTATCCBCGBacillus Calmette–Guérin - Bacillus Calmette-GuerinBSA - BSAbovine serum albumin - آلبومین سرم گاوCancer testis antigen - آنتی ژن بیضه سرطانCancer-testis antigen - آنتی ژن سرطانی بیضهimmunotherapy - ایمونوتراپیIHC - ایمونوهیستوشیمیImmunohistochemistry - ایمونوهیستوشیمیTransurethral resection of bladder tumor - برداشتن تراکم تومور مثانهWorld Health Organization - سازمان بهداشت جهانیUrinary bladder cancer - سرطان مثانه ادرارPeptide vaccines - واکسن های پپتیدquantitative real-time PCR - واکنش زنجیره ای پلیمراز واقعی در زمان واقعیTransitional cell carcinoma - کارسینوم سلول گذارWHO - که
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Frequent expression of zinc-finger protein ZNF165 in human urinary bladder transitional cell carcinoma Frequent expression of zinc-finger protein ZNF165 in human urinary bladder transitional cell carcinoma](/preview/png/10940879.png)
چکیده انگلیسی
The aim of the study is to evaluate mRNA/protein expression of zinc finger protein 165 (ZNF165) in transitional cell carcinomas (TCCs) of urinary bladder and correlate its expression with the clinicopathological characteristics of patients. In this study, the methods of quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry (IHC) were utilized to evaluate mRNA/protein expression of ZNF165 in TCC. Independent Student's t test, ANOVA and Chi-square (Ï2) were used to analyze the data statistically. We observed overexpression of ZNF165 mRNA in testis and majority (59.2%) of TCC patients. ZNF165 mRNA expression was also detected in adjacent noncancerous tissues (ANCTs) and some other normal tissues. Relative mean fold expression of ZNF165 mRNA was found to be significantly (p < 0.01) higher in muscle-invasive bladder cancer (MIBC) as compared to non-muscle-invasive bladder cancer (NMIBC) patients. (12.11 ± 9.57 vs. 5.72 ± 2.61, p = 0.009). ZNF165 protein expression was demonstrated on archival formalin-fixed, paraffin-embedded (FFPE) bladder tissues using IHC and nuclear staining pattern was detected. No significant difference was observed in protein expression of ZNF165 between the two groups (NMIBC and MIBC patients) (61.1% vs. 55.2%, p = 0.629). No significant protein expression of ZNF165 was observed among ANCTs and benign prostatic hyperplasia (BPH) used as control. Our study results suggest that ZNF165 mRNA/protein expression was observed in TCC of human urinary bladder and might be used as a novel diagnostic biomarker and as well a vaccine target in development of urinary bladder cancer specific immunotherapy.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Immunobiology - Volume 220, Issue 1, January 2015, Pages 68-73
Journal: Immunobiology - Volume 220, Issue 1, January 2015, Pages 68-73
نویسندگان
Pankaj Kumar Singh, Anupam Kumar Srivastava, Divakar Dalela, Srikanta Kumar Rath, Madhu Mati Goel, Madan Lal Brahma Bhatt,