کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10941014 | 1095548 | 2013 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
IL-1β and TGFβ2 synergistically induce endothelial to mesenchymal transition in an NFκB-dependent manner
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کلمات کلیدی
ECMGAPDHHUVECeNOSαSMAEndMTIKK-2DMSO - DMSOEndothelial to mesenchymal transition - Endothelial به انتقال مزانشیمیα-smooth muscle actin - اکتین عضله آلفا صافDimethylsulfoxide - دیمتیل سولفواکسیدHuman umbilical vein endothelial cells - سلول های اندوتلیالی ورید ناف انسانendothelial nitric oxide synthase - سنتاز اکسید نیتریک اندوتلیالglyceraldehyde 3-phosphate dehydrogenase - گلیسرولیدید 3-فسفات دهیدروژناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Endothelial to mesenchymal transition (EndMT) contributes to fibrotic diseases. The main inducer of EndMT is TGFβ signaling. TGFβ2 is the dominant isoform in the physiological embryonic EndMT, but its role in the pathological EndMT in the context of inflammatory co-stimulation is not known. The aim of this study was to investigate TGFβ2-induced EndMT in the context of inflammatory IL-1β signaling. Co-stimulation with IL-1β and TGFβ2, but not TGFβ1, caused synergistic induction of EndMT. Also, TGFβ2 was the only TGFβ isoform that was progressively upregulated during EndMT. External IL-1β stimulation was dispensable once EndMT was induced. The inflammatory transcription factor NFκB was upregulated in an additive manner by IL-1β and TGFβ2 co-stimulation. Co-stimulation also led to the nuclear translocation of NFκB which was sustained over long-term treatment. Activation of NFκB was indispensable for the co-induction of EndMT. Our data suggest that the microenvironment at the verge between inflammation (IL-1β) and tissue remodeling (TGFβ2) can strongly promote the process of EndMT. Therefore our findings provide new insights into the mechanisms of pathological EndMT.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Immunobiology - Volume 218, Issue 4, April 2013, Pages 443-454
Journal: Immunobiology - Volume 218, Issue 4, April 2013, Pages 443-454
نویسندگان
Monika Maleszewska, Jan-Renier A.J. Moonen, Nicolette Huijkman, Bart van de Sluis, Guido Krenning, Martin C. Harmsen,