کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10959691 1100541 2011 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Adhesive cell cultivation on polymer particle having grafted epoxy polymer chain
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
Adhesive cell cultivation on polymer particle having grafted epoxy polymer chain
چکیده انگلیسی
In this study, we synthesized a new cell immobilization support having poly(glycidyl methacrylate) as a graft polymer chain and used this support for cell cultivation. Base polymer particle was synthesized by suspension polymerization and epoxy polymer chain was extended from particle surface on graft polymerization. Produced polymer particles had broad particle size distribution ranging from 20 to 1000 μm and the degree of polymerization of grafted polymer chain was ranged from 500 to 1000. The effects of various factors, such as grafted polymer chain length and its surface density, composition of base polymer network and graft polymer chain, on the cell growth of murine fibroblast cell line (MS-5 cell) on polymer particle were studied. This polymer particle could cultivate not only fibroblast cell line but also epidermal cell line (HeLa cell), osteoblast cell line (MC3T3E1 cell), and chondrocyte cell line (ch-8 cell) on its surface. Growth rate is almost the same as that of cells using poly(styrene) tissue culture dish. To apply this cell cultivation system for examination of cell co-culture, HeLa cell immobilized on 100 μm of polymer particle was successfully co-cultured with MS-5 cell immobilized on 300 μm of polymer particle for four weeks.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Tissue and Cell - Volume 43, Issue 2, April 2011, Pages 115-124
نویسندگان
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