Generation of heterozygous and homozygous hESC H9 sublines carrying inactivating mutations in RB1

Resource tableUnique stem cell lines identifierWAe009-A-12WAe009-A-13Alternative names of stem cell linesC7 (homozygous deletion, WAe009-A-12)G12LS (heterozygous deletion, WAe009-A-13)InstitutionUniversity Hospital Essen, University Duisburg-Essen, Essen, GermanyContact information of distributorDr. Laura Steenpass, laura.steenpass@uni-due.deDr. Deniz Kanber, deniz.kanber@uni-due.deType of cell linesESCOriginHumanCell SourceHuman ESC line H9 purchased from WiCellClonalityClonalMethod of reprogrammingN/AMultiline rationaleclones selected for deletion in heterozygous and homozygous stateGene modificationYESType of modificationIndels in RB1 exon 3Associated diseaseRetinoblastomaGene/locusRB1, chromosome 13q14.2Method of modificationCRISPR/Cas9 nucleaseName of transgene or resistanceN/AInducible/constitutive systemN/ADate archived/stock dateC7 12.05.2018G12LS 12.05.2018Cell line repository/bankN/AEthical approvalApproval obtained from the Robert-Koch Institute, Berlin, Germany (Az.3.04.02/0101) and from the local Ethical Review Board University Duisburg-Essen (16-7215-BO)