کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
11022627 | 1701476 | 2019 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
miR-181a regulate porcine preadipocyte differentiation by targeting TGFBR1
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
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چکیده انگلیسی
miRNAs have been shown to regulate a variety of biological process. It has been shown that miR-181a regulates porcine adipogenesis by targeting Tumor Necrosis Factor-α (TNF-α), but the overall functions of miR-181a in porcine preadipocyte differentiation remain unclear. This study aimed to explore the functions of miR-181a in porcine preadipocyte differentiation via the TGFβ/Smad pathway. The TargetScan program was used to predict miRNAs targeting TGFBR1, and miR-181a was selected as a candidate. To investigate the functions of miR-181a, miRNA mimics and inhibitors were used to overexpress or knockdown miR-181a, respectively. RT-qPCR and Western blotting were used to measure the expression of aP2, PPARγ, C/EBPα and TGFBR1 in porcine preadipocytes. Lipid accumulation and adipocyte apoptosis were detected using Oil Red O staining and flow cytometry, respectively. Taken together, our results indicated that miR-181a promoted porcine preadipocyte differentiation by directly targeting TGFBR1.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 681, 10 January 2019, Pages 45-51
Journal: Gene - Volume 681, 10 January 2019, Pages 45-51
نویسندگان
Zhe Zhang, Yan Gao, Ming-Qiang Xu, Chang-Jiang Wang, Xu-Huang Fu, Jian-Bo Liu, Dong-Xu Han, Hao Jiang, Bao Yuan, Jia-Bao Zhang,