کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
11023205 | 1701328 | 2018 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Ion chromatographic method for the determination of orotic acid in urine
ترجمه فارسی عنوان
روش کروماتوگرافی یونی برای تعیین اسید اورورتیک در ادرار
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کلمات کلیدی
اسید اورتریک کروماتوگرافی یونی، اختلال متابولیک، نقص چرخه اوره، اسیدوریا آرژنین ادرار
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Excess urinary orotic acid excretion occurs in patients with some inborn errors of metabolic pathways such as pyrimidine synthesis and urea cycle. Thus, rapid diagnosis of orotic aciduria has a vital importance for patients. In this paper, a novel method for determination of orotic acid in urine samples by ion chromatography with suppressed conductivity detection was investigated. The separation of orotic acid from urine matrix was accomplished by using an anion exchange column with optimized isocratic eluent program which utilized 50â¯mM NaOH. The other chromatographic conditions were as follows: the suppressor current was 31â¯mA; the flow rate of mobile phase was 0.25â¯mLâ¯minâ1; the column temperature was 30â¯Â°C; sample loop volume was 10â¯Î¼L. Under optimized conditions, the limit of detection was 0.2â¯Î¼molâ¯Lâ¾1. Dramatically elevated orotic acid concentration was observed on pathological urine samples comparing to healthy urines, as expected. There are a good many advantages of the proposed method, but using an environmentally friendly reagent free system, no organic solvent employment and its quick nature, and being a sensitive and reliable method are the most obvious ones. The proposed method, therefore, may be utilized as an alternative technique for clinical laboratories.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 563, 15 December 2018, Pages 9-14
Journal: Analytical Biochemistry - Volume 563, 15 December 2018, Pages 9-14
نویسندگان
Orhan DestanoÄlu, Damla Zeydanlı, Mehmet Åerif Cansever, Gülçin GümüÅ Yılmaz,