کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
11029929 | 1646409 | 2018 | 21 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Design of oligonucleotide-capped mesoporous silica nanoparticles for the detection of miRNA-145 by duplex and triplex formation
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
The development of new strategies to detect microRNAs (miRNAS) has become an important challenge in the biomedical field. We report herein the use of oligonucleotide-gated silica nanoparticles for the detection of miRNA-145. In the proposed design, mesoporous silica nanoparticles (MSNs) are loaded with a fluorescent reporter (rhodamine B) and pores are blocked by specific DNA oligonucleotides. The opening of the gated system and dye delivery is selectively controlled by DNA-miRNA recognition. Moreover, the use of DNA capture probes able to form duplex or triplex structures between target miRNA and the complementary oligonucleotides has been studied. By this simple procedure a limit of detection as low as 0.25âpM was found for miRNA. The method was successfully applied to detect miRNA-145 in serum samples, which demonstrates the high potential of these capped materials to detect miRNA for diagnostic purposes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 277, 20 December 2018, Pages 598-603
Journal: Sensors and Actuators B: Chemical - Volume 277, 20 December 2018, Pages 598-603
نویسندگان
Ãngela Ribes, Sara Santiago-Felipe, Anna Aviñó, Vicente Candela-Noguera, Ramón Eritja, Félix Sancenón, Ramón MartÃnez-Máñez, Elena Aznar,