کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1173430 1491370 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Immobilized trypsin on hydrophobic cellulose decorated nanoparticles shows good stability and reusability for protein digestion
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Immobilized trypsin on hydrophobic cellulose decorated nanoparticles shows good stability and reusability for protein digestion
چکیده انگلیسی

The preparation of biocatalysts based on immobilized trypsin is of great importance for both proteomic research and industrial applications. Here, we have developed a facile method to immobilize trypsin on hydrophobic cellulose-coated silica nanoparticles by surface adsorption. The immobilization conditions for the trypsin enzyme were optimized. The as-prepared biocatalyst was characterized by Fourier transform infrared spectroscopy, transmission electron microscopy, and elemental analysis. In comparison with free enzyme, the immobilized trypsin exhibited greater resistances against thermal inactivation and denaturants. In addition, the immobilized trypsin showed good durability for multiple recycling. The general applicability of the immobilized trypsin for proteomic studies was confirmed by enzymatic digestion of two widely used protein substrates: bovine serum albumin (BSA) and cytochrome c. The surface adsorption protocols for trypsin immobilization may provide a promising strategy for enzyme immobilization in general, with great potential for a range of applications in proteomic studies.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 477, 15 May 2015, Pages 21–27
نویسندگان
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