کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1173571 1491383 2014 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Tag/hybridization-based sensitive detection of polymerase chain reaction products
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Tag/hybridization-based sensitive detection of polymerase chain reaction products
چکیده انگلیسی

The polymerase chain reaction (PCR) is an important technology to amplify a single copy or a few copies of DNA segment in genomic DNAs, visualizing the segment as DNA fragment. Thus, PCR is frequently used in various examinations such as detection of bacteria and fungi in the food industry. Here, we report a simple and sensitive method for detection of PCR products using single-strand tag sequence and hybridization of the tag sequence to the complementary tag sequence immobilized on solid material (STH). The detection sensitivity was found to be at least 50 times higher than electrophoresis/ethidium bromide (EtBr) visualization for approximately a 500-bp fragment and higher than the ordinary hybridization, that is, hybridization of denatured PCR product to probe sequence immobilized on solid material.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 464, 1 November 2014, Pages 12–16
نویسندگان
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