کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1173573 1491383 2014 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Spectrophotometric determination and removal of unchelated europium ions from solutions containing Eu-diethylenetriaminepentaacetic acid chelate–peptide conjugates
ترجمه فارسی عنوان
تعیین اسپکتروفتومتریک و حذف یونهای یوروپیم بدون رژیم از محلولهایی که حاوی اتیلین تری اتیلینتریپینات سیتی اسید کلاته پپتید
کلمات کلیدی
اروپائیوم، کلاته، زایولنول نارنجی، امپور، چلکس
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی

Europium chelates conjugated with peptide ligands are routinely used as probes for conducting in vitro binding experiments. The presence of unchelated Eu ions in these formulations gives high background luminescence and can lead to poor results in binding assays. In our experience, the reported methods for purification of these probes do not achieve adequate removal of unchelated metal ions in a reliable manner. In this work, a xylenol orange-based assay for the quantification of unchelated metal ions was streamlined and used to determine levels of metal ion contamination as well as the success of metal ion removal on attempted purification. We compared the use of Empore chelating disks and Chelex 100 resin for the selective removal of unchelated Eu ions from several Eu-diethylenetriaminepentaacetic acid chelate–peptide conjugates. Both purification methods gave complete and selective removal of the contaminant metal ions. However, Empore chelating disks were found to give much higher recoveries of the probes under the conditions used. Related to the issue of probe recovery, we also describe a significantly more efficient method for the synthesis of one such probe using Rink amide AM resin in place of Tentagel S resin.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 464, 1 November 2014, Pages 24–29
نویسندگان
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