کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1173636 1491395 2014 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Enhanced assay of endothelial exocytosis using extracellular matrix components
ترجمه فارسی عنوان
تست پیشرفته اگزوسیتوز اندوتلیال با استفاده از اجزای ماتریکس خارج سلولی
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی

Vascular inflammation plays a key role in the pathogenesis of atherosclerosis. The first step in vascular inflammation is endothelial exocytosis, in which endothelial granules fuse with the plasma membrane, releasing prothrombotic and proinflammatory messenger molecules. The development of cell culture models to study endothelial exocytosis has been challenging because the factors that modulate exocytosis in vitro are not well understood. Here we report a method for studying endothelial exocytosis that optimizes extracellular matrix components, cell density, and duration of culture. Human umbilical vein endothelial cells plated on collagen I-coated plates and cultured in the confluent state for 7–12 days in low-serum medium showed robust secretion of von Willebrand factor when stimulated with various agonists. This exocytosis assay is rapid and applicable to high-throughput screening.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 452, 1 May 2014, Pages 19–24
نویسندگان
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