کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1174181 961734 2012 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Measurement of marine osmolytes in mammalian serum by liquid chromatography–tandem mass spectrometry
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Measurement of marine osmolytes in mammalian serum by liquid chromatography–tandem mass spectrometry
چکیده انگلیسی

Osmolytes are accumulated intracellularly to offset the effects of osmotic stress and protect cellular proteins against denaturation. Because different taxa accumulate different osmolytes, they can also be used as “dietary biomarkers” to study foraging. Potential osmolyte biomarkers include glycine betaine, trimethylamine N-oxide (TMAO), homarine, dimethylsulfoniopropionate (DMSP), and the osmolyte analog arsenobetaine (AsB). We present a liquid chromatography–tandem mass spectrometry (LC–MS/MS) assay for the simultaneous measurement of these osmolytes in serum or plasma. Varying concentrations of osmolytes were added to serum and samples and extracted in 90% acetonitrile and 10% methanol containing 10 μM deuterated internal standards (D9-glycine betaine, D9-trimethylamine-N-oxide, 13C2-arsenobetaine, D6-DMSP, and D4-homarine). Analytes were separated on a normal-phase modified silica column and detected using isotope dilution tandem mass spectrometry in multiple reaction monitoring (MRM) mode. The assay was linear for all six compounds (r2 values = 0.983–0.996). Recoveries were greater than 85%, and precision for within-batch coefficients of variation (CVs) were less than 8.2% and between-batch CVs were less than 6.1%. Limits of detection ranged from 0.02 to 0.12 μmol/L. LC–MS/MS is a simple method with high throughput for measuring low levels of osmolytes that are often present in biological samples.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 420, Issue 1, 1 January 2012, Pages 7–12
نویسندگان
, , ,