کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1174279 961739 2011 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Protein chip for the parallel quantification of high and low abundant biomarkers for sepsis
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Protein chip for the parallel quantification of high and low abundant biomarkers for sepsis
چکیده انگلیسی

We present herein a protein chip for diagnosis of sepsis that combines both a sandwich and a binding inhibition format in order to quantify high (CRP) and low abundant proteins (cytokines, PCT, neopterin) in parallel. Using the combined assay format the lowest detectable concentrations for CRP, IL-6, IL-8, IL-10, TNFα, PCT, and neopterin are 3 mg/L, 15 ng/L, 26 ng/L, 65 ng/L, 40 ng/L, 78 ng/L, and 0.46 μg/L. Four different combined assay formats are tested, using separate or joint incubation steps of analytes and detection antibodies. Yet, low limit of detection (LOD) and short processing time are contradictory: while the combined assay performed in a multistep protocol is extremely sensitive (e.g., the LOD for IL-6 is 15 ng/L), but more time-consuming (4 h), the all-in-one protocol takes only 2.5 h, but suffers from lower sensitivity compared with the multistep protocol (e.g., the LOD for IL-6 is up to 40 times enhanced). Reproducibility is good in both cases (CV 5–20%).

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 419, Issue 1, 1 December 2011, Pages 46–52
نویسندگان
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