کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1174451 961751 2010 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Therapeutic antibodies: Discovery and development using the ProteOn XPR36 biosensor interaction array system
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Therapeutic antibodies: Discovery and development using the ProteOn XPR36 biosensor interaction array system
چکیده انگلیسی

Therapeutic monoclonal antibodies are becoming a significant and rapidly growing class of therapeutic pharmaceuticals. Their discovery and development requires fast and high-throughput methodologies for screening and selecting appropriate candidate antibodies having high affinity for the target as well as high specificity and low cross-reactivity. This study demonstrates the use of the ProteOn XPR36 protein interaction array system and its novel approach, termed One-Shot Kinetics, for the rapid screening and selection of high-affinity antibodies. This approach allows multiple quantitative protein binding analyses in parallel, providing association, dissociation, and affinity constants for several antibodies or supernatants simultaneously in one experiment. We show that the ProteOn XPR36 system is a valuable tool for use across multiple stages of the therapeutic antibody discovery and development process, enabling efficient and rapid screening after panning, affinity maturation, assay validation, and clone selection.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 406, Issue 2, 15 November 2010, Pages 147–156
نویسندگان
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