کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1174477 961753 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Polyaniline–carbon nanotube composite film for cholesterol biosensor
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Polyaniline–carbon nanotube composite film for cholesterol biosensor
چکیده انگلیسی

Nanocomposite film composed of polyaniline (PANI) and multiwalled carbon nanotubes (MWCNT), prepared electrophoretically onto indium tin oxide (ITO)-coated glass plate, was used for covalent immobilization of cholesterol oxidase (ChOx) via N-ethyl-N′-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) chemistry. Results of linear sweep voltammetric measurements reveal that ChOx/PANI–MWCNT/ITO bioelectrode can detect cholesterol in the range of 1.29 to 12.93 mM with high sensitivity of 6800 nA mM−1 and a fast response time of 10 s. Photometric studies for ChOx/PANI–MWCNT/ITO bioelectrode indicate that it is thermally stable up to 45 °C and has a shelf life of approximately 12 weeks when stored at 4 °C. The results of these studies have implications for the application of this interesting matrix (PANI–MWCNT) toward the development of other biosensors.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 383, Issue 2, 15 December 2008, Pages 194–199
نویسندگان
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