کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1174652 961762 2011 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Determination of complementary antibody pairs using protein A capture with the AlphaScreen assay format
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Determination of complementary antibody pairs using protein A capture with the AlphaScreen assay format
چکیده انگلیسی

The utility of antibody reagents for the detection of specific cellular targets for both research and diagnostic applications is widespread and continually expanding. Often it is useful to develop specific antibodies as reagent pairs that distinguish different epitopes of the target such that sandwich enzyme-linked immunosorbent assay can be used for selective and specific detection. However, the identification of pairing antibodies is often cumbersome and labor-intensive even with the use of designed peptide-specific epitopes as antigens. We have developed a robust and high-throughput method for identifying pairing complementary antibodies derived either from commercial sources or during a rabbit hybridoma monoclonal screening and selection process using protein A capture with the AlphaScreen bead-based assay format. We demonstrate the value and effectiveness of this assay with three protein targets: Akt2, ATF3, and NAEβ (the β-subunit of the neddylation activation enzyme).

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 408, Issue 2, 15 January 2011, Pages 321–327
نویسندگان
, , , , , , , , , , ,