کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1174653 961762 2011 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Sodium dodecyl sulfate–agarose gel electrophoresis for the detection and isolation of amyloid curli fibers
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Sodium dodecyl sulfate–agarose gel electrophoresis for the detection and isolation of amyloid curli fibers
چکیده انگلیسی

Curli are amyloid-like fibers on the surface of some strains of Escherichia coli and Salmonella enteritidis. We tested the use of horizontal sodium dodecyl sulfate (SDS)–agarose gel electrophoresis to detect, isolate, and quantitate curli. Cell extracts fractionated in SDS–agarose gels and stained with Coomassie blue exhibited a soluble fraction that entered the gel and an insoluble fraction that remained in the well. Much more insoluble material was observed with curli-proficient strains than with strains that do not make curli. Both highly purified curli and the insoluble material isolated from an SDS–agarose gel could be dissociated into monomers when treated with formic acid. For quantitation, we immobilized samples in SDS–agarose prior to electrophoresis. This avoids losses during the staining of the gel. Our methods provide a rapid and simple fractionation of curli using equipment that is readily available.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 408, Issue 2, 15 January 2011, Pages 328–331
نویسندگان
, , ,