کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1174774 961768 2010 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Improving the sensitivity of matrix-assisted laser desorption/ionization (MALDI) mass spectrometry by using polyethylene glycol modified polyurethane MALDI target
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Improving the sensitivity of matrix-assisted laser desorption/ionization (MALDI) mass spectrometry by using polyethylene glycol modified polyurethane MALDI target
چکیده انگلیسی

Previous studies in our group have shown that the analyte signal in a matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) experiment is strongly influenced by the binding interactions between the target surface and the analyte. Specifically, the analyte signal increases with decreases in surface binding affinity, which has been attributed to more unbound analyte being available for incorporation within the MALDI matrix. In this work, polyethylene glycol (PEG) was chemically grafted onto a polyurethane (PU) film to produce a MALDI target having reduced surface–protein binding affinity, and the effect of this modification on protein MALDI ion signals was investigated. The proteins myoglobin, lysozyme, and albumin were used to evaluate the PEG PU modified target as compared with a PU target and a commercial stainless steel target. It is shown that there are enhancements in the protein MALDI ion signals on the PEG PU modified target and that the limit of detection for these proteins is decreased by a factor of 2 to 6 in comparison with the unmodified PU and the commercial stainless steel targets.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 400, Issue 1, 1 May 2010, Pages 56–60
نویسندگان
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