کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1174778 | 961768 | 2010 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterization of trisulfide modification in antibodies
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Characterization of trisulfide modification in antibodies Characterization of trisulfide modification in antibodies](/preview/png/1174778.png)
چکیده انگلیسی
Trisulfides are a posttranslational modification formed by the insertion of a sulfur atom into a disulfide bond. Although reports for trisulfides in proteins are limited, we find that they are a common modification in natural and recombinant antibodies of all immunoglobulin G (IgG) subtypes. Trisulfides were detected only in interchain linkages and were predominantly in the light–heavy linkages. Factors that lead to trisulfide formation and elimination and their impact on activity and stability were investigated. The peptide mapping methods developed for characterization and quantification of trisulfides should be applicable to any antibody and can be easily adapted for other types of proteins.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 400, Issue 1, 1 May 2010, Pages 89–98
Journal: Analytical Biochemistry - Volume 400, Issue 1, 1 May 2010, Pages 89–98
نویسندگان
Sheng Gu, Dingyi Wen, Paul H. Weinreb, Yaping Sun, Lihe Zhang, Susan F. Foley, Rashmi Kshirsagar, David Evans, Sha Mi, Werner Meier, R. Blake Pepinsky,