کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1174864 961772 2010 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Fullerol–fluorescein isothiocyanate phosphorescent labeling reagent for the determination of glucose and alkaline phosphatase
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Fullerol–fluorescein isothiocyanate phosphorescent labeling reagent for the determination of glucose and alkaline phosphatase
چکیده انگلیسی

The active –OH group in fullerol (F-ol) could react with the dissociated –COOH group in fluorescein isothiocyanate (FITC) to form F-ol–(FITC)n, which could emit room temperature phosphorescence (RTP) signal of F-ol and FITC on acetate cellulose membrane (ACM), respectively. Their RTP signals were enhanced by N,N-dimethylaniline (DMA). The labeling reaction between the –NCS group of FITC in DMA–F-ol–(FITC)n and the –NH2 group in wheat germ agglutinin (WGA) produced DMA–F-ol–(FITC)n–WGA, which could further take affinity adsorption (AA) reaction with bioactive substances (BS), such as glucose and alkaline phosphatase (AP), to produce DMA–F-ol–(FITC)n–WGA–BS. Both of these two products could maintain the good RTP characteristics of F-ol and FITC. Based on the facts above, a new phosphorescent labeling reagent, DMA–F-ol–FITC, was developed, and a new affinity adsorption solid substrate room temperature phosphorimetry (AASSRTP) for the determination of BS was established. This method was applied to the determination of BS in human serum and the diagnosis of diseases, with the results agreeing very well with those of enzyme-linked immunosorbent assay (ELISA). The mechanism of DMA–F-ol–(FITC)n labeling of WGA and AASSRTP for the determination of BS is discussed.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 404, Issue 2, 15 September 2010, Pages 223–231
نویسندگان
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