کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1175343 | 961797 | 2010 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Fluorescence intensity- and lifetime-based glucose sensing using an engineered high-Kd mutant of glucose/galactose-binding protein Fluorescence intensity- and lifetime-based glucose sensing using an engineered high-Kd mutant of glucose/galactose-binding protein](/preview/png/1175343.png)
We synthesized mutants of glucose/galactose-binding protein (GBP), labeled with the environmentally sensitive fluorophore Badan, with the aim of producing a fluorescence-based glucose sensing system with an operating range compatible with continuous glucose monitoring in patients with diabetes mellitus. From five mutants tested, the triple mutant H152C/A213R/L238S–Badan showed a large (200%) maximal increase in fluorescence intensity on the addition of glucose, with a binding constant (Kd) of 11 mM, an operating range of approximately 1–100 mM, and similar responses in buffer and serum. The mean fluorescence lifetime of this mutant also increased by 70% on the addition of glucose. We conclude that the GBP mutant H152C/A213R/L238S, when labeled with Badan, is suitable for development as a robust sensor for in vivo glucose monitoring in diabetes.
Journal: Analytical Biochemistry - Volume 399, Issue 1, 1 April 2010, Pages 39–43