کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1175429 | 961801 | 2009 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Heart-cut capillary electrophoresis for drug analysis in mouse blood with electrochemical detection
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Heart-cut capillary electrophoresis for drug analysis in mouse blood with electrochemical detection Heart-cut capillary electrophoresis for drug analysis in mouse blood with electrochemical detection](/preview/png/1175429.png)
چکیده انگلیسی
A novel separation method, which interfaced capillary zone electrophoresis (CZE) and micellar electrokinetic capillary chromatography (MEKC), was developed for analysis of weak basic compounds. CZE was used as the first separation, from which the effluent components were transferred and further analyzed by MEKC. As the key to successful integration of CZE and MEKC, a new microhole interface was designed. On-line concentration strategies, namely pH junction and sweeping, were employed to avoid sample zone diffusion at the interface. For evaluation of the feasibility and effectiveness of the capillary electrophoresis (CE) system, basic cardiovascular drugs were used as model compounds. The resulting electropherogram was quite different from that of either CZE or MEKC separation. The relative standard deviations (RSDs) of peak height, peak area, and migration time were in the ranges of 2.7% to 4.5%, 1.3% to 3.6%, and 0.8% to 1.4%, respectively, and detection limits (signal/noise = 3) were 0.015 to 0.052 mg/L. The proposed method was successfully applied to the determination of basic cardiovascular drugs in mouse blood.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 387, Issue 2, 15 April 2009, Pages 171-177
Journal: Analytical Biochemistry - Volume 387, Issue 2, 15 April 2009, Pages 171-177
نویسندگان
Zhao-Xiang Zhang, Xiao-Wei Zhang, Shu-Sheng Zhang,