کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1175472 961803 2008 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Reversible detection of proteases and their inhibitors by a pulsed chronopotentiometric polyion-sensitive electrode
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Reversible detection of proteases and their inhibitors by a pulsed chronopotentiometric polyion-sensitive electrode
چکیده انگلیسی

Polymer membrane electrodes operated by pulsed chronopotentiometry have recently been introduced to replace traditional ion-selective electrodes for a number of applications. While ion-selective electrodes for the polycation protamine have been reported, for instance, a pulsed chronopotentiometric readout mode (called here pulstrode) provides improved stability and reproducibility while exhibiting sufficient selectivity for the direct detection of protamine in undiluted whole blood samples. Here, such protamine-sensitive pulstrodes are applied for the real-time detection of the activity of the protease trypsin and its soybean inhibitor. This is possible because small fragments produced by the trypsin digestion are not detectable by the protamine-sensing membrane. The real-time response to the proteolytic reaction is shown to exhibit good reproducibility and reversibility, and the initial reaction rate is dependent on the concentration of the protease and its inhibitor.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 374, Issue 2, 15 March 2008, Pages 366–370
نویسندگان
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