کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1175554 961806 2009 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Quantitation of proteins using a dye–metal-based colorimetric protein assay
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Quantitation of proteins using a dye–metal-based colorimetric protein assay
چکیده انگلیسی

We describe a dye–metal (polyhydroxybenzenesulfonephthalein-type dye and a transition metal) complex-based total protein determination method. The binding of the complex to protein causes a shift in the absorption maximum of the dye–metal complex from 450 to 660 nm. The dye–metal complex has a reddish brown color that changes to green on binding to protein. The color produced from this reaction is stable and increases in a proportional manner over a broad range of protein concentrations. The new Pierce 660 nm Protein Assay is very reproducible, rapid, and more linear compared with the Coomassie dye-based Bradford assay. The assay reagent is room temperature stable, and the assay is a simple and convenient mix-and-read format. The assay has a moderate protein-to-protein variation and is compatible with most detergents, reducing agents, and other commonly used reagents. This is an added advantage for researchers needing to determine protein concentrations in samples containing both detergents and reducing agents.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 385, Issue 2, 15 February 2009, Pages 342–345
نویسندگان
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