کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1176134 | 1491414 | 2006 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Quantitation of protein on gels and blots by infrared fluorescence of Coomassie blue and Fast Green
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Quantitation of protein on gels and blots by infrared fluorescence of Coomassie blue and Fast Green Quantitation of protein on gels and blots by infrared fluorescence of Coomassie blue and Fast Green](/preview/png/1176134.png)
چکیده انگلیسی
Coomassie blue staining of gels and blots is commonly employed for detection and quantitation of proteins by densitometry. We found that Coomassie blue or Fast Green FCF bound to protein fluoresces in the near infrared. We took advantage of this property to develop a rapid and sensitive method for detection and quantitation of proteins in gels and on blots. The fluorescence response is quantitative for protein content between 10 ng and 20 μg per band or spot. Staining and destaining require only 30 min, and the method is compatible with subsequent immunodetection.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 350, Issue 2, 15 March 2006, Pages 233–238
Journal: Analytical Biochemistry - Volume 350, Issue 2, 15 March 2006, Pages 233–238
نویسندگان
Shen Luo, Nancy B. Wehr, Rodney L. Levine,