کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1176222 961837 2007 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Affinity profiling using the peptide microarray technology: A case study
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Affinity profiling using the peptide microarray technology: A case study
چکیده انگلیسی
Little about the reliability of measurements obtained using synthetic peptide microarrays is known. We report results from a study on the quantitative reliability of microarrays manufactured by robot-supported immobilization of presynthesized peptides for different microarray platforms. Technological precision is assessed for inter- and intra-device readout comparisons. Correlations between measured signals and known dissociation constants using a phenomenological model derived from the mass action law are discussed. Special emphasis is on discussing the pitfalls of high-throughput affinity measurements. We show that the quantitative determination of binding affinities is prone to be biased toward a mean affinity of around 10−7 M, while the classification of peptides into either “binders” or “nonbinders” provides very high prediction accuracy. The experimental requirements needed to obtain reliable binding affinity predictions are discussed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 363, Issue 1, 1 April 2007, Pages 108-118
نویسندگان
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