کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1176222 | 961837 | 2007 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Affinity profiling using the peptide microarray technology: A case study
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
Little about the reliability of measurements obtained using synthetic peptide microarrays is known. We report results from a study on the quantitative reliability of microarrays manufactured by robot-supported immobilization of presynthesized peptides for different microarray platforms. Technological precision is assessed for inter- and intra-device readout comparisons. Correlations between measured signals and known dissociation constants using a phenomenological model derived from the mass action law are discussed. Special emphasis is on discussing the pitfalls of high-throughput affinity measurements. We show that the quantitative determination of binding affinities is prone to be biased toward a mean affinity of around 10â7Â M, while the classification of peptides into either “binders” or “nonbinders” provides very high prediction accuracy. The experimental requirements needed to obtain reliable binding affinity predictions are discussed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 363, Issue 1, 1 April 2007, Pages 108-118
Journal: Analytical Biochemistry - Volume 363, Issue 1, 1 April 2007, Pages 108-118
نویسندگان
Victor Tapia, Juliane Bongartz, Mike Schutkowski, Nicole Bruni, Armin Weiser, Bernhard Ay, Rudolf Volkmer, Michal Or-Guil,