کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1176387 961848 2008 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Aptamer biosensor for protein detection using gold nanoparticles
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Aptamer biosensor for protein detection using gold nanoparticles
چکیده انگلیسی

Combining gold nanoparticles (GNPs) as fluorescence quencher and aptamer as probe, we have developed protein biosensors by using DNA-modified GNPs. We examined how the experimental design, such as the type of interaction between DNA strands and GNPs, temperature, and microenvironment of aptamer, influences the recognition ability of the biosensor. Under our experimental conditions, the recognition of protein by the complex of dye-labeled DNA hybridized with aptamer that is immobilized on GNPs (Ap–Im–GNPs) shows the best character in protein detection.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 373, Issue 2, 15 February 2008, Pages 213–219
نویسندگان
, , , ,