کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1176618 961865 2012 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Performance characteristics of monoclonal antibodies as recyclable binders to cardiac troponin I
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Performance characteristics of monoclonal antibodies as recyclable binders to cardiac troponin I
چکیده انگلیسی

Acute myocardial infarction is a typical disorder that requires continuous monitoring for early detection of potential life-threatening situations. To this end, we used different methods to screen for rapidly reversible antibodies, among 22 hybridoma clones, against cardiac troponin I (cTnI), which is a specific marker indicating the disease. The dissociation rates of antibodies were underestimated by up to a factor of 1000 because of bivalent binding when tested with the antigen immobilized on solid surfaces. This effect was also observed in a sandwich immunoassay, in which the detection antibody cross-linked with various antigen molecules already bound to the capture antibody. Although multiple binding events contributed to enhanced detection capability, it was difficult to recycle the immunosensor. We then devised a screening system by arranging the test antibody for the capture binder immobilized on a label-free sensor. This enabled us to select fast reactive antibodies of which one (clone 24) was shown to be recyclable, even in serum-containing medium. Using this antibody, repetitive detection of cTnI with a rapid response time (half-life of dissociation: about 4 min on average) and high detection capability (0.1 ng/ml) was achieved, which is very important for detection in a clinical setting.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 431, Issue 1, 1 December 2012, Pages 11–18
نویسندگان
, , , , ,