کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1176994 961921 2010 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A red line not to cross: Evaluating the limitation and properness of gel image tuning procedures
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
A red line not to cross: Evaluating the limitation and properness of gel image tuning procedures
چکیده انگلیسی
Currently, results of gel electrophoresis are commonly documented in digital formats by image acquisition instruments. In this study, gel images tuned by a common image processing software package, Photoshop, were assessed to understand the transforming algorithms and their impacts on quantitative analysis. TotalLab 100, an electrophoresis gel image analysis software package, was applied for image quantitation and evaluation. The three most frequently used image tuning functions-adjustments of the brightness, contrast, and grayscale span (level) of images-were investigated using both data generated from a standard grayscale tablet and an actual electrophoresis gel image. The influences of these procedures were analyzed for the grayscale transformation between the input and output images. Although all three procedures differentially improved the visualization of the input image, adjusting the contrast of images disrupted the quantitative information because of its nonlinear transforming algorithm. Under certain conditions, adjusting the brightness or the level of images could preserve the quantitative information because of the linear transforming algorithms. It was found that when the minimum and maximum grayscales of a gel image were recognized, using a commercial software package to maximally stretch the level may significantly improve the quality of a gel image without jeopardizing quantitative analysis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 396, Issue 1, 1 January 2010, Pages 42-50
نویسندگان
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