کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1177061 961937 2009 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Measurement of enzyme kinetics and inhibitor constants using enthalpy arrays
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Measurement of enzyme kinetics and inhibitor constants using enthalpy arrays
چکیده انگلیسی

Enthalpy arrays enable label-free, solution-based calorimetric detection of molecular interactions in a 96-detector array format. Compared with conventional calorimetry, enthalpy arrays achieve a significant reduction of sample volume and measurement time through the combination of the small size of the detectors and ability to perform measurements in parallel. The current capabilities of the technology for studying enzyme-catalyzed reactions are demonstrated by determining the kinetic parameters for reactions with three model enzymes. In addition, the technology has been used with two classes of enzymes to determine accurate inhibitor constants for competitive inhibitors from measurements at a single inhibitor concentration.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 388, Issue 2, 15 May 2009, Pages 204–212
نویسندگان
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