کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1177161 1491411 2008 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Two-step purification of low-content cellular protein using protein-imprinted polymers
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Two-step purification of low-content cellular protein using protein-imprinted polymers
چکیده انگلیسی
We introduce a new method, based on molecular imprinting, for purification of low-content cellular protein. This is a combination method that uses two types of protein-imprinting polymers (PIPs) synthesized with limited-length polymer chains that contain randomly distributed recognition sites, namely assistant recognition polymer chains, and uses cloned bacterial protein as a template. The low-content cellular target protein was purified from cell extract by this method. This is believed to be the first time that low-content cellular protein has been purified by using PIPs and with only two steps.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 380, Issue 2, 15 September 2008, Pages 268-275
نویسندگان
, , , , , , ,