کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1177178 961957 2008 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Label-free optical detection of aptamer–protein interactions using gold-capped oxide nanostructures
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Label-free optical detection of aptamer–protein interactions using gold-capped oxide nanostructures
چکیده انگلیسی

Optical biosensors based on noble nanostructures currently receive attention due to their highly efficient, simultaneous analysis of a number of important biomolecules from proteomics to genomics. In this study, the combination of localized surface plasmon resonance (LSPR) with interferometry in the relative reflected intensity (RRI) spectrum of the gold-capped oxide nanostructure was thoroughly exploited for label-free detection of aptamer–protein interactions. The fabrication of gold-capped oxide nanostructure involved the deposition of gold on the surface of porous anodic alumina (PAA) layer chip. This novel nanomaterial enabled us to simultaneously monitor the changes in both LSPR and interferometric characteristics since the biomolecular interactions occur. After immobilizing the aptamer I on the chip surface, our sensor could be easily applied for specific detection of thrombin and aptamer II with a limit of detection of 1 nM thrombin in the sample. Our optical biosensing device connecting with the gold-capped oxide nanostructure has a high potential for highly sensitive monitoring of the other biomolecular interactions such as protein–protein interactions, DNA–protein interactions, DNA–DNA hybridizations, and ligand–receptor interactions with a massively parallel detection capability in a high-throughput system.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 379, Issue 1, 1 August 2008, Pages 1–7
نویسندگان
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