کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1177322 961982 2007 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A universal plate format for increased throughput of assays that monitor multiple aminoacyl transfer RNA synthetase activities
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
A universal plate format for increased throughput of assays that monitor multiple aminoacyl transfer RNA synthetase activities
چکیده انگلیسی

Aminoacyl transfer RNA (tRNA) synthetases are intensely studied enzymes because of their importance in the establishment of the genetic code and their connection to disease and medicine. During the advancement of this field, several assays were developed. Despite many innovations, the sensitivity, simplicity, and reliability of the radiometric assays (which were among the first to be developed) have ensured their continued use. Four activities are measured by these assays: active site titration, amino acid activation, aminoacylation, and posttransfer editing (deacylation). In an effort to maintain the advantage of these assays while enhancing throughput, reducing waste, and improving data quality, a universal 96-well filter plate format was developed. This format facilitates the assays for all four of the widely studied activities.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 368, Issue 1, 1 September 2007, Pages 111–121
نویسندگان
, , , , ,