کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1177354 | 961992 | 2007 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
DNA entrapped polypyrrole-polyvinyl sulfonate film for application to electrochemical biosensor
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
Double-stranded calf thymus (dsCT)-DNA was electrochemically entrapped into polypyrrole-polyvinyl sulfonate (PPy-PVS) films deposited onto indium tin oxide (ITO) coated glass plates. These dsCT-DNA entrapped PPy-PVS/ITO films were characterized using cyclic voltammetry, UV-visible, Fourier transform infrared (FT-IR), scanning tunneling microscopy (STM), and electrochemical impedance measurements. Attempts made to use these dsCT-DNA entrapped PPy-PVS/ITO films for detection of 2-aminoanthracene (0.001-6.0 ppm) and 3-chlorophenol (0.01-55.0 ppm) revealed a response time of 30 s and a shelf life of approximately 25 weeks when stored under desiccated conditions at 25 °C. The addition of salts such as Ca2+ (250 ppm), Mg2+ (200 ppm), Clâ (1560 ppm), and Na+ (150 ppm) ions contained in water does not affect the observed amperometric response of the disposable dsCT-DNA entrapped PPy-PVS film-based electrochemical biosensor.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 366, Issue 1, 1 July 2007, Pages 71-79
Journal: Analytical Biochemistry - Volume 366, Issue 1, 1 July 2007, Pages 71-79
نویسندگان
Nirmal Prabhakar, Kavita Arora, Surinder P. Singh, Harpal Singh, Bansi D. Malhotra,