کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1177520 962021 2006 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A rapid and efficient method for purification of recombinant adenovirus with arginine–glycine–aspartic acid-modified fibers
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
A rapid and efficient method for purification of recombinant adenovirus with arginine–glycine–aspartic acid-modified fibers
چکیده انگلیسی

Recombinant adenoviral vectors (adenovectors) have been subject to various genetic modifications to improve their transduction efficiency and targeting capacity. Production and purification of adenovectors with modified capsid proteins can be problematic using conventional two-cycle CsCl gradient ultracentrifugation. We have developed a new method for purifying recombinant adenovectors in two steps: iodixanol discontinuous density gradient ultracentrifugation and size exclusion column chromatography. The purity and infectious activity of adenovectors isolated by the two methods were comparable. The new method yielded three to four times more adenovectors with arginine–glycine–aspartic acid (RGD)-modified fiber proteins than did the conventional CsCl method. For other fiber-modified and wild-type adenovectors, the yields of the two methods were comparable. Thus, the iodixanol-based method can be used not only to improve the production of RGD-modified adenovectors but also to purify adenovectors with or without fiber modifications. Moreover, the whole procedure can be completed in 3 h. Therefore, this method is rapid and efficient for production of recombination adenovectors, especially those with RGD-modified fibers.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 354, Issue 1, 1 July 2006, Pages 140–147
نویسندگان
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