کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1177531 962026 2006 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Validation of the drop coating deposition Raman method for protein analysis
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Validation of the drop coating deposition Raman method for protein analysis
چکیده انگلیسی

Drop coating deposition Raman (DCDR) spectroscopy is critically evaluated to establish the limits to which it may be used to detect changes in protein conformation, binding, and purity. Difference spectroscopy is used to evaluate the reproducibility of the DCDR spectra under various experimental conditions. The results indicate (i) the absence of thermal/photochemical laser damage induced by the Raman excitation laser under typical DCDR data collection conditions, (ii) the reproducibility of DCDR spectra from samples with different volumes or concentrations, (iii) the water content of DCDR protein deposits and associated spectral signatures, and (iv) the degree of similarity between solution Raman spectra and DCDR spectra.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 353, Issue 2, 15 June 2006, Pages 157–166
نویسندگان
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