کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1193049 | 1492258 | 2014 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Comparison of VUV radiation induced fragmentation of thymidine and uridine nucleosides – The effect of methyl and hydroxyl groups Comparison of VUV radiation induced fragmentation of thymidine and uridine nucleosides – The effect of methyl and hydroxyl groups](/preview/png/1193049.png)
• Uridine fragments more extensively than thymidine.
• The majority of uridine fragments include the uracil moiety.
• The majority of thymidine fragments include the deoxyribose moiety.
• Uridine has more fragments with an intact glycosidic bond than thymidine.
• The hydroxyl group affects the fragmentation more than the methyl group.
Fragmentation of thymidine and uridine nucleosides followed by vacuum ultraviolet radiation was compared using time-of-flight (TOF) mass spectroscopy. TOF spectra of 5-methyluridine were also measured in order to investigate the effect of the 5-methyl group of the base and the 2′-hydroxyl group of the sugar moiety on the fragmentation. It was observed that uridine fragments more extensively than thymidine and that the base moiety fragments were more abundant in uridine. It was concluded that the extent and the site of fragmentation are influenced more by the 2′-hydroxyl group of the sugar moiety than the methyl group of the base. It was also observed that the methyl and hydroxyl groups together have a combined effect of stabilizing the nucleoside in respect to thermal dissociation upon vaporization.
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Journal: International Journal of Mass Spectrometry - Volume 370, 15 September 2014, Pages 96–100