Back to deuterium: Utility of 2H-labeled peptides for targeted quantitative proteomics

► 2H- and 13C/15N-based peptides are comparable as quantitation standards for targeted mass spectrometry. ► Precision and accuracy for MRM-MS quantitation are not affected by the chromatographic isotope effect of 2H-based peptides. ► Cost benefit of 2H-based peptides allows for large-scale applications in targeted quantitative proteomics.