کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1193769 | 1492349 | 2008 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Fragmentation of phosphorylated and singly charged peptide ions via interaction with metastable atoms Fragmentation of phosphorylated and singly charged peptide ions via interaction with metastable atoms](/preview/png/1193769.png)
Fragmentation of phosphorylated peptide ions via interaction with electronically excited metastable argon atoms was studied in a linear trap—time-of-flight mass spectrometer. Doubly charged ions of phosphorylated peptides from an Enolase digest were produced by electrospray ionization and subjected to a metastable atom beam in the linear trap. The metastable argon atoms were generated using a glow-discharge source. An intensive series of c- and z-ions were observed in all cases, with the phosphorylation group intact. The formation of molecular radical cations with reduced charge indicated that an electron transfer from a highly excited metastable state of argon to the peptide cation occurred. Additionally, singly charged Bradykinin, Substance P and Fibrinopeptide A molecular ions were fragmented via interaction with electronically excited metastable helium atoms. The fragmentation mechanism was different in this case and involved Penning ionization.
Journal: International Journal of Mass Spectrometry - Volume 278, Issues 2–3, 1 December 2008, Pages 150–157