کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1193796 | 1492320 | 2010 | 6 صفحه PDF | دانلود رایگان |
Lithiated glucose-containing disaccharides with various linkages and anomeric configurations, formed by electrospray ionization (ESI) and trapped in a Fourier transform ion cyclotron resonance (FTICR) mass spectrometer, were fragmented by infrared radiation from a tunable CO2 laser. Irradiation over the wavelength range from 9.2 to 9.7 μm gave unique fragmentation patterns for each of the disaccharides. These fragmentation patterns can be used to easily determine the monosaccharide linkage position for each disaccharide. Once the linkage is determined, irradiation of the precursor ion (m/z 349) to produce a specific ratio of peak height for a particular fragment ion to that of the precursor ion is shown to yield unique ratio values that can be used to identify the different anomers of the same linkage at the 95% confidence level.
Wavelength-dependent fragmentation patterns of lithiated, glucose-containing disaccharides, produced with a tunable CO2 laser, can be used to distinguish both linkage position and anomeric configurations of the disaccharides.Figure optionsDownload high-quality image (83 K)Download as PowerPoint slide
Journal: International Journal of Mass Spectrometry - Volume 297, Issues 1–3, 1 November 2010, Pages 96–101