Hydrogen/deuterium exchange reflects binding of human centrin 2 to Ca2+ and Xeroderma pigmentosum Group C peptide: An example of EX1 kinetics

Xeroderma pigmentosum (XP) is a genetic disease affecting 1 in 10,000–100,000 and predisposes people to early-age skin cancer, a disease that is increasing. Those with XP have decreased ability to repair UV-induced DNA damage, leading to increased susceptibility of cancerous non-melanomas and melanomas. A vital, heterotrimeric protein complex is linked to the nucleotide excision repair pathway for the damaged DNA. The complex consists of XPC protein, human centrin 2, and RAD23B. One of the members, human centrin 2, is a ubiquitous, acidic, Ca2+-binding protein belonging to the calmodulin superfamily. The XPC protein contains a sequence motif specific for binding to human centrin 2. We report here the Ca2+-binding properties of human centrin 2 and its interaction with the XPC peptide motif. We utilized a region-specific H/D exchange protocol to localize the interaction of the XPC peptide with the C-terminal domain of centrin, the binding of which is different than that of calmodulin complexes. The binding dynamics of human centrin 2 to the XPC peptide in the absence and presence of Ca2+ are revealed by the observation of EX1 H/D exchange regime, indicating that a locally unfolded population exists in solution and undergoes fast H/D exchange.

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► Kinetics of H/DX of centrin 2 was measured by proteolysis and mass spectrometry.
► Kinetics shows the site of interaction of the protein with the XPC peptide.
► The kinetics constitutes an unusual example of EX1 kinetics of H/DX.
► Local unfolding of centrin/XPC gives a species that undergoes fast H/DX.