کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1194264 | 1492350 | 2008 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Fragmentation of Lewis-type trisaccharides in the gas phase: Experimental and theoretical studies
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The mechanisms of fragmentation of the chlorinated Lewis-type trisaccharides Lewis a and Lewis X were studied by using experimental and theoretical methods. In the fragment ion spectra obtained by ESI-CID-MS, C- and Z-type fragmentations corresponding to 4- and 3-linked glycosyl bond cleavages of the N-acetylglucosamine moiety were observed. The relationships of the relative ion intensities of C- and Z-type fragmentations were ZÂ >Â C in Lewis a and CÂ >Â Z in Lewis X, suggesting that the relationships of the activation energies were CÂ >Â Z in Lewis a and ZÂ >Â C in Lewis X. Anomeric and acetoamide groups were assumed to be included in the mechanisms of C- and Z-type fragmentation; we therefore analyzed 3-fucosyllactose and methylated Lewis-type trisaccharides, which are the derivatives of Lewis-type trisaccharides. These analyses revealed that C-type fragmentation was little influenced by anomeric group and that Z-type fragmentation was influenced by both the anomeric and the acetoamide groups. Fragmentation mechanisms were proposed on the basis of the experimental results and were calculated at the HF/3-21G(d), HF/6-31G(d) levels. From the calculations, C-type fragmentation of the 4-linkage was considered to take place by electron transfer from the acetoamide group, and Z-type fragmentation at the 3-linkage was considered to take place in two steps: (i) elimination of the 3-linked saccharide moiety, and (ii) deprotonation of an anomeric proton by a chloride anion. The activation energies for C- and Z-type fragmentations, as determined by HF calculations, were consistent with the experimentally assumed trends.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Mass Spectrometry - Volume 278, Issue 1, 15 November 2008, Pages 1-9
Journal: International Journal of Mass Spectrometry - Volume 278, Issue 1, 15 November 2008, Pages 1-9
نویسندگان
Hiroaki Suzuki, Tohru Yamagaki, Kazuo Tachibana, Kazuhiko Fukui,