کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1194364 1492303 2011 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Structural characterization of cyanidin-3,5-diglucoside and pelargonidin-3,5-diglucoside anthocyanins: Multi-dimensional fragmentation pathways using high performance liquid chromatography-electrospray ionization-ion trap-time of flight mass spectrometry
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Structural characterization of cyanidin-3,5-diglucoside and pelargonidin-3,5-diglucoside anthocyanins: Multi-dimensional fragmentation pathways using high performance liquid chromatography-electrospray ionization-ion trap-time of flight mass spectrometry
چکیده انگلیسی

Cyanidin-3,5-diglucoside and pelargonidin-3,5-diglucoside were identified from an extract of freeze-dried red hybrid-tea rose petals with high mass accuracy and multi-dimensional fragmentation using liquid chromatography-electrospray ionization-ion trap-time of flight mass spectrometry (LC-ESI-IT-TOF-MS). The effects of varying the amount of collision energy applied to the trap at each MS stage were studied to optimize subsequent fragmentation steps. Structural characterization of cyanidin and pelargonidin was attempted by identifying the fragments created by cross-ring cleavage (CRC) of the C-ring and small neutral molecule loss. These fragments were further determined to be diagnostic for differentiating the compounds of interest. A computational study of the CRC fragments of cyanidin was performed in order to calculate the overall energy of the fragments. It was determined that the 0,2 CRC pathway was more favorable than the 0,3 CRC pathway.

Figure optionsDownload high-quality image (248 K)Download as PowerPoint slideHighlights
► Anthocyanin diglucosides were identified from a rose using LC-ESI-IT-TOF-MS.
► The collision energy of the trap at each MS stage was optimized for fragmentation.
► Diagnostic fragments from cross-ring cleavage and small molecule loss were identified.
► The compounds were differentiated based on their higher order fragmentation patterns.
► A computational study was used to further characterize fragmentation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Mass Spectrometry - Volume 308, Issue 1, 1 November 2011, Pages 71–80
نویسندگان
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