Practical utilization of nanocrystal metal organic framework biosensor for parathion specific recognition

• Luminescent nanocrystal metal organic framework (NMOF1) has been used for biosensing of parathion.
• The –COOH pendent group of organic linker on the surface of luminescent NMOF1 has been activated.
• The fluorescent studies revealed successful interaction of anti-parathion antibody with parathion
• Luminescent NMOF1 sensor allowed the detection of parathion in the range of 1 ppb to 1 ppm.
• Developed biosensor is highly stable and sensitive for specific recognition of parathion.

The feasibility of the nanocrystal metal organic framework [Cd(atc)(H2O)2]n (NMOF1) as a biosensor is explored for the specific recognition of parathion. The luminescence properties of the NMOF1/antiparathion complex were tested for the detection of parathion. Because of the fluorescent properties, the anti-parathion antibody interacted with parathion in order to facilitate its detection at a dynamic concentration range from 1 ppb to 1 ppm. The assay was validated against some environmental samples by gas chromatography with solid phase extraction (SPE). Accordingly, the NMOF biosensor tested in this work is demonstrated as parathion-specific with high surface loading and high sensitivity.