کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1227928 | 968440 | 2010 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Laccase–polyazetidine prepolymer–MWCNT integrated system: Biochemical properties and application to analytical determinations in real samples Laccase–polyazetidine prepolymer–MWCNT integrated system: Biochemical properties and application to analytical determinations in real samples](/preview/png/1227928.png)
Bioelectrochemical properties of Trametes versicolor Laccase (TvL) and Trametes hirsuta Laccase (ThL) immobilized by using polyazetidine prepolymer (PAP) onto multi-walled carbon nanotubes (MWCNTs) screen printed electrode (SPE) surface, have been studied with several redox mediators by cyclic voltammetry (CV). The efficient entrapment of laccase in the PAP layer was confirmed by determination of both kinetic parameters (maximum current and Michaelis–Menten apparent constant) and analytical performances by chronoamperometry. The Laccase-modified MWCNTs electrode provides an effective biosensor for determination of polyphenols and catecholamines in real matrices; performances of the considered biosensors for real samples analysis are also compared and discussed.
Journal: Microchemical Journal - Volume 96, Issue 2, November 2010, Pages 301–307